| Autor: |
S, Wielders, M, Mukherjee, J, Michiels, D T, Rijkers, J P, Cambus, R W, Knebel, V, Kakkar, H C, Hemker, S, Béguin |
| Rok vydání: |
1997 |
| Předmět: |
|
| Zdroj: |
Thrombosis and haemostasis. 77(4) |
| ISSN: |
0340-6245 |
| Popis: |
The area under the thrombin generation curve (the endogenous thrombin potential; ETP) has been proposed as a parameter for plasma-based hypercoagulability and to monitor anticoagulant treatment. We present an ETP assay for the routine laboratory using a centrifugal analyser. Throughput is 30 samples/h, within and between run imprecision is 4-5.6%. Suitable substrates were developed for the ranges of 10-500% and 2-100% of normal. Independent of tissue factor concentration (if4 pM), the normal value of the extrinsic ETP is 384.8 +/- 51.7 nM.min. The intrinsic ETP, triggered by ellagic acid, is 414 +/- 41 nM.min. The ETP is decreased to 15 and 35% of normal by oral anticoagulation (INR 2.5-4.0) and by heparin administration (APTT 1.5-2.5 x control). The ETP is increased in untreated subjects with congenital antithrombin deficiency and in women using oral contraceptives. In deep vein thrombosis (phlebographically confirmed), it is increased by 29.4% (extrinsic) and 53% (intrinsic). In (angiographically assessed) coronary artery disease the increase is by 10% and 17% respectively. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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