Regulation of RAW264.7 macrophage polarization on smooth and rough surface topographies by galectin-3
| Autor: | F, Kianoush, M, Nematollahi, J D, Waterfield, D M, Brunette |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Surface Properties
Galectin 3 Macrophages Morpholines Cell Polarity Nitric Oxide Synthase Type II Lactose Receptors Cell Surface Mice Phosphatidylinositol 3-Kinases Imaging Three-Dimensional Mannose-Binding Lectins RAW 264.7 Cells Gene Expression Regulation Chromones Animals Lectins C-Type Interleukin-4 Cell Shape Mannose Receptor Phosphoinositide-3 Kinase Inhibitors |
| Zdroj: | Journal of biomedical materials research. Part A. 105(9) |
| ISSN: | 1552-4965 |
| Popis: | Recognition of topographical features induces phenotypic changes in macrophages although the receptors and signaling pathways are not completely characterized. As integrin molecules in focal adhesions/podosomes are in intimate contact with topography and topography modulates the NFkB pathway through cholesterol enriched raft-associated adhesive signaling structures we hypothesized that a cell-surface signaling complex comprised of galectin-3 together with its ligand CD98 and integrinβ1 is important for topography-directed lineage determination. This study used polished, sand blasted and acid etched (SLA) surfaces and two novel grooved topographies (G1 and G2) produced by anisotropic etching of Si1 1 0to evaluate the role of galectin-3 in macrophage polarization in RAW 264.7 macrophages, as determined by gene expression and morphology. In the presence of the galectin-3 inhibitor, lactose, the M2 marker (mannose receptor) was down-regulated while the M1 marker (iNOS) was up-regulated on smooth and rough surfaces. This skewing of phenotype suggests a role for galectin-3 in macrophage polarization towards the M2 phenotype. Additionally, we evaluated the role of PI3K on polarization using PI3K inhibitor LY294002. We found that the M2 marker was down-regulated on both PO (surface polished) and G1 surfaces implicating PI3K in lineage determination. We also found that surface topography altered cell morphology; macrophages had a larger area on G2 surfaces. Lactose treatment significantly reduced the cell area on all topographies suggesting that the galectin-3 is also involved in signaling complexes triggering the rearrangement of the actin cytoskeleton. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2499-2509, 2017. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text