Effects of metalloprotease inhibitors on the conversion of proendothelin-1 to endothelin-1
| Autor: | A J, Trapani, J L, Balwierczak, R W, Lappe, J L, Stanton, S C, Graybill, M F, Hopkins, P, Savage, D M, Sperbeck, A Y, Jeng |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Thiorphan Dose-Response Relationship Drug Endothelin-1 Swine Endothelins Glycopeptides Metalloendopeptidases Blood Pressure Benzazepines Endothelin-Converting Enzymes In Vitro Techniques Coronary Vessels Muscle Smooth Vascular Rats Rats Sprague-Dawley Methionine Animals Aspartic Acid Endopeptidases Neprilysin Endothelium Vascular Propionates Protein Precursors Muscle Contraction |
| Zdroj: | Biochemistry and molecular biology international. 31(5) |
| ISSN: | 1039-9712 |
| Popis: | The IC50 values of phosphoramidon, CGS 25015, CGS 26129, thiorphan and benazeprilat for inhibition of endothelin converting enzyme partially purified from porcine aortic endothelial cells were 3.5, 18, 58,100 and100 microM, respectively. A similar rank order of potency was observed for inhibition of the proendothelin-1 (proET-1) -induced pressor response in the rat where phosphoramidon, CGS 25015, CGS 26129, thiorphan and benazeprilat at 30 mg/kg i.v. produced 65, 57, 27, 12, and 0% inhibition, respectively. A slightly different rank order of potency was obtained in the proET-induced contraction of porcine coronary arteries where IC50 values of10, 10-30, 10-30, 30-100 and 30-100 microM were exhibited by CGS 25015, CGS 26129, phosphoramidon, thiorphan and benazeprilat, respectively. These data indicate that the endothelin converting enzymes in the three systems studied are similar, except that phosphoramidon is a slightly more potent inhibitor in the in vitro assay and the in vivo pressor test than in the smooth muscle contraction assay. |
| Databáze: | OpenAIRE |
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