Comparison of cobas
| Autor: | A Lum, Han, Hak Ryul, Kim, Keum Ha, Choi, Ki Eun, Hwang, Mengyu, Zhu, Yuya, Huang, Moxin, Wu, Young Jin, Lee, Min Cheol, Park, Ji Hyun, Cho, Do Sim, Park |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Aged 80 and over Male cobas EGFR Mutation test v2 PANAMutyper-R-EGFR Genotype Epidermal growth factor receptor Pleural effusion supernatant DNA Middle Aged Polymerase Chain Reaction ErbB Receptors Pleural Effusion Young Adult Plasma Mutation Humans Female Original Article Reagent Kits Diagnostic Diagnostic Genetics Aged |
| Zdroj: | Annals of Laboratory Medicine |
| ISSN: | 2234-3814 |
| Popis: | Background Plasma epidermal growth factor receptor (EGFR) mutation tests are less invasive than tissue EGFR mutation tests. We determined which of two kits is more efficient: cobas EGFR Mutation test v2 (cobasv2; Roche Molecular Systems, Pleasanton, CA, USA) or PANAMutyper-R-EGFR (Mutyper; Panagene, Daejeon, Korea). We also evaluated whether pleural effusion supernatant (PE-SUP) samples are assayable, similar to plasma samples, using these two kits. Methods We analyzed 156 plasma and PE-SUP samples (31 paired samples) from 116 individuals. We compared the kits in terms of accuracy, assessed genotype concordance (weighted κ with 95% confidence intervals), and calculated Spearman's rho between semi-quantitatively measured EGFR-mutant levels (SQIs) measured by each kit. We also compared sensitivity using 47 EGFR-mutant harboring samples divided into more-dilute and less-dilute samples (dilution ratio: ≥ or |
| Databáze: | OpenAIRE |
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