| Autor: |
Ting-Cai, Cheng, Ren-Wen, Long, Yu-Qian, Wu, You-Bing, Guo, Duo-Lian, Liu, Li, Peng, Dai-Qin, Li, Dai-Wen, Yang, Xin, Xu, Feng-Xiang, Liu, Qing-You, Xia |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Insect science. 23(3) |
| ISSN: |
1744-7917 |
| Popis: |
Tarantula venoms provide a model system for studying toxin selectivity, structure-activity relationships and molecular evolution of peptide toxins. Previous studies have identified a large number of peptide toxins in the venom of the Chinese bird spider Haplopelma hainanum, generally regarded as a highly venomous spider. However, the lack of available RNA-seq transcriptomic and genomic data is an obstacle to understanding its venom at the molecular level. In this study, we investigated the venom gland transcriptome of H. hainanum by RNA-seq, in the absence of an available genomic sequence. We identified 201 potential toxins among 57 181 de novo assembled transcripts, including knottins, Kunitz-type toxins, enzymes and other proteins. We systematically identified most of the knottins and Kunitz-type toxins, some of which showed strongly biased expression in the venom gland, including members of the huwentoxin-1, huwentoxin-2 and magi-1 families. We also discovered several novel potential toxins. These data demonstrate the high molecular and structural diversity in the venom toxins of H. hainanum. This study offers a useful strategy for exploring the complex components of spider venoms. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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