| Autor: |
G J, van Kamp, G G, Bon, R A, Verstraeten, D, Lynch, M, Krikau, J, Flückiger, A, Ruibal, P, Kenemans |
| Rok vydání: |
1996 |
| Předmět: |
|
| Zdroj: |
Clinical chemistry. 42(1) |
| ISSN: |
0009-9147 |
| Popis: |
We describe a new fully automated procedure for the quantitative measurement of CA 15-3: the microparticle enzyme immunoassay (MEIA) technology developed by Abbott Labs. for the IMx automated immunoassay analyzer. The new IMx CA 15-3 test uses two mouse monoclonal antibodies, 115D8 and DF3. The test has a dynamic range to 250 kilounits/L and a minimal detectable dose of CA 15-30.2 kilounits/L. On dilution, linearity is excellent, with recoveries ranging from 94% to 101%. Studies were conducted at four sites to evaluate the performance characteristics of this assay. The intra- and interassay CVs were4.7% and5.6%, respectively, and showed a between-laboratory CV5.9%. Test results of the Abbott IMx CA 15-3 (y) were correlated with those obtained with the Centocor CA 15-3 RIA (x), a solid-phase heterologous RIA. Linear regression analysis on results for 1973 samples yielded: y = 0.97x - 2.09 (r = 0.9899, Sy/x = 22.2, range 1-4089 kilounits/L). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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