Improvement of Duchenne muscular dystrophy phenotype following obestatin treatment
| Autor: | Jessica, González-Sánchez, Agustín, Sánchez-Temprano, Tania, Cid-Díaz, Regina, Pabst-Fernández, Carlos S, Mosteiro, Rosalía, Gallego, Ruben, Nogueiras, Xesús, Casabiell, Gillian S, Butler-Browne, Vincent, Mouly, José Luis, Relova, Yolanda, Pazos, Jesús P, Camiña |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Duchenne muscular dystrophy Muscle Fibers Skeletal Skeletal muscle Receptors G-Protein-Coupled Mice Sarcolemma Obestatin signalling Animals Humans Muscle Strength Muscle Skeletal Original Articles Ghrelin Rats Muscular Dystrophy Duchenne Disease Models Animal Phenotype Pharmacological modifier Protein Biosynthesis Skeletal muscle cell atrophy Proteolysis Mice Inbred mdx Original Article Oxidation-Reduction Biomarkers |
| Zdroj: | Journal of Cachexia, Sarcopenia and Muscle |
| ISSN: | 2190-6009 |
| Popis: | Background This study was performed to test the therapeutic potential of obestatin, an autocrine anabolic factor regulating skeletal muscle repair, to ameliorate the Duchenne muscular dystrophy (DMD) phenotype. Methods and results Using a multidisciplinary approach, we characterized the ageing‐related preproghrelin/GPR39 expression patterns in tibialis anterior (TA) muscles of 4‐, 8‐, and 18‐week‐old mdx mice (n = 3/group) and established the effects of obestatin administration at this level in 8‐week‐old mdx mice (n = 5/group). The findings were extended to in vitro effects on human immortalized DMD myotubes. An analysis of TAs revealed an age‐related loss of preproghrelin expression, as precursor of obestatin, in mdx mice. Administration of obestatin resulted in a significant increase in tetanic specific force (33.0% ± 1.5%, P |
| Databáze: | OpenAIRE |
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