Rabbit model of
| Autor: | Oren, Gordon, Robert J, Miller, John M, Thompson, Alvaro A, Ordonez, Mariah H, Klunk, Dustin A, Dikeman, Daniel P, Joyce, Camilo A, Ruiz-Bedoya, Lloyd S, Miller, Sanjay K, Jain |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Staphylococcus aureus Lumbar Vertebrae Prosthesis-Related Infections Time Factors Implant-associated infection Bone Screws Post-surgical infection Spinal infection Staphylococcal Infections Proof of Concept Study Bacterial Load Disease Models Animal PET Biofilms Positron Emission Tomography Computed Tomography Animals Orthopedic Procedures Bone Remodeling Microscopy Electrochemical Scanning Rabbits Resource Article Bioluminescence Bone Plates |
| Zdroj: | Disease Models & Mechanisms article-version (VoR) Version of Record |
| ISSN: | 1754-8411 |
| Popis: | Post-surgical implant-associated spinal infection is a devastating complication commonly caused by Staphylococcus aureus. Biofilm formation is thought to reduce penetration of antibiotics and immune cells, contributing to chronic and difficult-to-treat infections. A rabbit model of a posterior-approach spinal surgery was created, in which bilateral titanium pedicle screws were interconnected by a plate at the level of lumbar vertebra L6 and inoculated with a methicillin-resistant S. aureus (MRSA) bioluminescent strain. In vivo whole-animal bioluminescence imaging (BLI) and ex vivo bacterial cultures demonstrated a peak in bacterial burden by day 14, when wound dehiscence occurred. Structures suggestive of biofilm, visualized by scanning electron microscopy, were evident up to 56 days following infection. Infection-induced inflammation and bone remodeling were also monitored using 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) and computed tomography (CT). PET imaging signals were noted in the soft tissue and bone surrounding the implanted materials. CT imaging demonstrated marked bone remodeling and a decrease in dense bone at the infection sites. This rabbit model of implant-associated spinal infection provides a valuable preclinical in vivo approach to investigate the pathogenesis of implant-associated spinal infections and to evaluate novel therapeutics. Summary: A model of post-surgical methicillin-resistant Staphylococcus aureus implant-associated spinal infection was created in rabbits, recapitulating acute infection as well as chronic low-burden infection, with structures suggestive of biofilm formation and bone remodeling. |
| Databáze: | OpenAIRE |
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