Peripheral-blood mononuclear cell responses to recombinant hepatitis C virus antigens in patients with chronic hepatitis C
| Autor: | H, Schupper, P, Hayashi, J, Scheffel, S, Aceituno, T, Paglieroni, P V, Holland, J B, Zeldis |
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| Rok vydání: | 1993 |
| Předmět: |
Adult
Antigens Differentiation T-Lymphocyte Male CD3 Complex Recombinant Fusion Proteins Viral Core Proteins Receptors IgG Hepacivirus Hepatitis C Antibodies Middle Aged Viral Nonstructural Proteins Lymphocyte Activation Hepatitis C CD56 Antigen Antigens CD Chronic Disease Leukocytes Mononuclear Humans Female Hepatitis Antibodies Hepatitis C Antigens Antigens Viral T-Lymphocytes Cytotoxic |
| Zdroj: | Hepatology (Baltimore, Md.). 18(5) |
| ISSN: | 0270-9139 |
| Popis: | Peripheral blood mononuclear cell proliferative responses in vitro to recombinant yeast or Escherichia coli hepatitis C virus fusion proteins were evaluated in 20 patients with chronic hepatitis C who were reactive for antibody to hepatitis C virus (on enzyme immunoassay, version 2.0, and a four-antigen recombinant immunoblot assay). Twenty age-matched, healthy individuals negative for antibody to hepatitis C virus were used as a control group. Peripheral-blood mononuclear cells from all chronic hepatitis C patients with antibodies to hepatitis C virus antigens c22 and c100-3 proliferated in vitro in response to the corresponding recombinant hepatitis C virus fusion protein. Peripheral-blood mononuclear cells from 75% of patients infected with hepatitis C virus proliferated in response to cytidine monophosphate-keto-3-deoxyoctulosonic acid-core recombinant antigen but there was no proliferative response to cytidine monophosphate-keto-3-deoxyoctulosonic acid-EF (derived from the NS5 region). All hepatitis C virus-infected patients had 33c antibody, but peripheral-blood mononuclear cells from only 9 of 14 (64%) proliferated in vitro in response to 33c. Ninety-five percent of all hepatitis C virus-infected patients had peripheral-blood mononuclear cells that proliferated in response to at least one recombinant hepatitis C virus fusion protein. The numbers and percentages of CD3 T cells, CD19 B cells and natural killer cells from patients with chronic hepatitis C virus infection did not differ from those in the healthy control group. However, the number of non-major histocompatibility complex-restricted cytotoxic T cells (CD3-positive, CD56-positive, CD16-positive) was increased in patients with chronic hepatitis C virus infection (p0.05). |
| Databáze: | OpenAIRE |
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