Determination of trimethoprim-sulfamethoxazole resistance in Streptococcus pneumoniae by using the E test with Mueller-Hinton agar supplemented with sheep or horse blood may be unreliable. The Pneumococcal Study Group

Autor:	M, Lovgren, L, Dell'Acqua, R, Palacio, G, Echániz-Aviles, A, Soto-Noguerón, E, Castañeda, C I, Agudelo, I, Heitmann, M C, Brandileone, R C, Zanella, A, Rossi, J, Pace, J A, Talbot
Rok vydání:	1998
Předmět:	Sheep Sulfamethoxazole Trimethoprim Resistance Bacteriology Microbial Sensitivity Tests Drug Resistance Multiple Trimethoprim Anti-Bacterial Agents Culture Media Blood Streptococcus pneumoniae Evaluation Studies as Topic Animals Humans Horses
Zdroj:	Journal of clinical microbiology. 37(1)
ISSN:	0095-1137
Popis:	An international, multicenter study compared trimethoprim-sulfamethoxazole MICs for 743 Streptococcus pneumoniae isolates (107 to 244 isolates per country) by E test, using Mueller-Hinton agar supplemented with 5% defibrinated horse blood or 5% defibrinated sheep blood, with MICs determined by the National Committee for Clinical Laboratory Standards broth microdilution reference method. Agreement within 1 log2 dilution and minor error rates were 69.3 and 15.5%, respectively, on sheep blood-supplemented agar and 76.9 and 13.6%, respectively, with horse blood as the supplement. Significant interlaboratory variability was observed. E test may not be a reliable method for determining the resistance of pneumococci to trimethoprim-sulfamethoxazole.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::63041e571aff397d9053d9569659a2f1 https://pubmed.ncbi.nlm.nih.gov/9854095 Zobrazit plný text záznamu