| Autor: |
Dragana, Robajac, Martina, Križáková, Miloš, Šunderić, Goran, Miljuš, Peter, Gemeiner, Olgica, Nedić, Jaroslav, Katrlík |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2460 |
| ISSN: |
1940-6029 |
| Popis: |
Lectin-based protein microarrays are used for glycoprofiling of various kinds of biological samples. Here we describe lectin-based microarray assay in the reverse-phase format where glycoprotein samples are spotted onto microarray slide and then are incubated with set of lectins. This configuration allows high-throughput screening of a large cohort of samples by a set of lectins without need of separation of glycans from glycoproteins. We applied the described method for glycan analysis of glycoprotein biomarkers of colorectal cancer associated with the insulin-like growth factor system. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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