Growth inhibition of prostate cancer by an adenovirus expressing a novel tumor suppressor gene, pHyde
| Autor: | M S, Steiner, X, Zhang, Y, Wang, Y, Lu |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Intrinsic Factor
Male DNA Complementary Avian Leukosis Virus Adenoviruses Human Molecular Sequence Data Mice Nude Prostatic Neoplasms Apoptosis Genetic Therapy Growth Inhibitors Rats Mice Adenovirus E3 Proteins Tumor Cells Cultured Adenovirus E1 Proteins Animals Humans Genes Tumor Suppressor Amino Acid Sequence Tumor Suppressor Protein p53 Promoter Regions Genetic |
| Zdroj: | Cancer research. 60(16) |
| ISSN: | 0008-5472 |
| Popis: | It has been estimated that there will be180,400 new cases of prostate cancer and 31,900 prostate cancer deaths in the United States this year. New therapeutic strategies against locally advanced prostate cancer are desperately needed. A novel gene (pHyde) was identified by an improved cDNA competition hybridization technique for Dunning rat prostate cancer cell lines. A recombinant replication-deficient E1/E3-deleted adenovirus type 5 containing a pHyde gene under the control of a truncated Rous sarcoma virus (RSV) promoter (AdRSVpHyde) was generated. In vitro, AdRSVpHyde significantly inhibited growth of human prostate cancer cell lines DU145 and LNCaP in culture. In vivo, a single injection of AdRSVpHyde (5 x 10(9) plaque-forming units) reduced DU145 tumors in nude mice remarkably compared with untreated control or viral control-treated DU145 tumors. Moreover, AdRSVpHyde induced apoptosis and stimulated p53 expression. These results together suggest that pHyde is a tumor suppressor gene that inhibits growth of prostate cancer and that this inhibition is at least in part due to the induction of apoptosis. |
| Databáze: | OpenAIRE |
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