Preparation of cross-linked enzyme aggregates of lipase from Aspergillus niger: process optimization, characterization, stability, and application for epoxidation of lemongrass oil
| Autor: | Abhijeet Bhimrao, Muley, Sneha, Awasthi, Prasanna Prakash, Bhalerao, Nilesh Lakshaman, Jadhav, Rekha Satishchandra, Singhal |
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| Rok vydání: | 2020 |
| Předmět: |
Terpenes
Temperature Lipase Hydrogen-Ion Concentration Enzymes Immobilized Culture Media Industrial Microbiology Kinetics Cross-Linking Reagents Glutaral Enzyme Stability Spectroscopy Fourier Transform Infrared Biocatalysis Epoxy Compounds Plant Oils Thermodynamics Aspergillus niger Particle Size Biotechnology Iodine |
| Zdroj: | Bioprocess and biosystems engineering. 44(7) |
| ISSN: | 1615-7605 |
| Popis: | Cross-linked enzyme aggregates (CLEAs) of lipase were prepared after fractional precipitation with 40-50% ammonium sulfate and then cross-linking with glutaraldehyde. The process variables for the preparation of lipase-CLEAs such as glutaraldehyde concentration, cross-linking period, and initial pH of medium were optimized. The optimized conditions for the preparation of lipase-CLEAs were 25 mM/80 min/pH 7.0, and 31.62 mM/90 min/pH 6.0 with one factor at a time approach and numerical optimization with central composite design, respectively. Lipase-CLEAs were characterized by particle size analysis, SEM, and FTIR. Cross-linking not only shifted the optimal pH and temperature from 7.0 to 7.5 and 40-45 to 45-50 °C, but also altered the secondary structure. Lipase-CLEAs showed an increase in K |
| Databáze: | OpenAIRE |
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