| Autor: |
Georgios, Vidalakis, Jinbo, Wang, Tyler, Dang, Tavia, Rucker, Sohrab, Bodaghi, Shi-Hua, Tan, Irene, Lavagi-Craddock, Alexandra, Syed, Gerardo, Uribe, Yi, Hsieh, Joana, Carbajal-Moreno |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2316 |
| ISSN: |
1940-6029 |
| Popis: |
Quantitative polymerase chain reaction (qPCR) and reverse transcription (RT)-qPCR have now become the gold standard for molecular diagnostics because of its sensitivity, specificity, and reproducibility. In addition, qPCR diagnostics are flexible because they can be scaled for high- or low-throughput applications. Here we describe an optimized assay and workflow for the universal detection of eight citrus viroid species and their variants by RT-qPCR. The assay allows for quick and efficient molecular detection of viroids without the need to run RT-qPCR for each individual viroid species. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
