HPAEC-PAD method for the analysis of alkaline hydrolyzates of Haemophilus influenzae type b capsular polysaccharide

Autor:	Alex, de Haan, Robert M F, van der Put, Michel, Beurret
Rok vydání:	2013
Předmět:	Sodium Acetate Hydrolysis Haemophilus influenzae type b Sodium Hydroxide Hydrogen-Ion Concentration Chromatography Ion Exchange Sensitivity and Specificity Bacterial Capsules Chromatography High Pressure Liquid Haemophilus Vaccines
Zdroj:	Biomedical chromatography : BMC. 27(9)
ISSN:	1099-0801
Popis:	A gradient method has been devised for the rapid analysis of alkaline hydrolyzates of Haemophilus influenzae type b (Hib) capsular polysaccharide-based vaccines by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). As compared with published procedures, peak shape and sensitivity were significantly improved with this approach, analysis time was short and there was little interference from impurities. The limits of detection and quantification were established with a purified reference polysaccharide. We propose this method as a practical alternative for the analysis of minute amounts of Hib polysaccharide, which can be lower than with the conventional approaches.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::5afbcc3ba6c627b72a97db5b88e85df1 https://pubmed.ncbi.nlm.nih.gov/23681893 Zobrazit plný text záznamu Plný text