| Autor: |
Hui-Hui, Wu, Tie-Qiang, Liu, Xue-Dong, Sun, Xiao-Mei, Huang, Rui, Zhang, Zhi-Qiang, Liu, Qiu-Hong, Man, Ya-Jing, Huang, Qi-Yun, Sun, Hong-Li, Zuo, Jian-Hui, Qiao, Chang-Lin, Yu, Kai-Xun, Hu, Hui-Sheng, Ai, Mei, Guo |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 22(3) |
| ISSN: |
1009-2137 |
| Popis: |
This study was purposed to establish and identify a H-2 completely mismatched microtransplantation model of leukemia mouse. The recipients were female BALB/c mice, while donors were male C57BL/6J mice. Recipients were inoculated intravenously with 1×10(6) of WEHI-3 cells, a cell line of myelomonocytic leukemia. Donors received 100 µg/kg G-CSF mobilization through hypodermic injection, every 12 hours, and it last 5 days. Chemotherapy regimens was MA (mitoxantrone+cytarabine), and it last 4 days. Recipients were given chemotherapy conditioning without GVHD prophylaxis after inoculation of leukemic cells for 2 days, and within 8 hours after last chemotherapy received donor mobilized spleen mononuclear cells (sMNC). The number of sMNC was (3, 6, 12) ×10(7), respectively. The early death rate, recovery level of WBC in peripheral blood and leukemia load were compared between chemotherapy and microtransplantation groups. The donor chimerism was detected by RT-PCR. From the clinical manifestation and pathological features, the GVHD in recipients was evaluated. The results showed that the early mortality in chemotherapy group was 25%, meanwhile those in the (3, 6, 12)×10(7) groups were 16.67%, 8.33%, 8.33%, respectively. The(3, 6)×10(7) groups has a stronger hematopoietic recovery capability than that in chemotherapy and 12×10(7) groups (P0.05) . There were more leukemic cells in chemotherapy mice than that in microtransplantation mice (P0.01) , and (12, 6)×10(7) groups had lower leukemia load than that in 3×10(7) group (P0.05) . No signs of GVHD were observed in microtransplantation mice. The donor microchimerism could be discovered at eraly 2 weeks after donor cell transfusion. It is concluded that a H-2 completely mismatched microtransplantation model of leukemia mouse has been successfully established, and it will provide a experimental base for studying microtransplantation in clinic. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
