[Effect of silencing LASP1 on biological behaviors of oral squamous cell carcinoma cells and IC50 of three anti-tumor drugs]
| Autor: | Qi, Chen, Xu, Wang, You-Cheng, Yu, Kui-Jie, Wei |
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| Rok vydání: | 2019 |
| Předmět: |
Mice
Nude Antineoplastic Agents Docetaxel LIM Domain Proteins Cytoskeletal Proteins Inhibitory Concentration 50 Mice Cell Movement Drug Resistance Neoplasm Cell Line Tumor Carcinoma Squamous Cell Animals Humans Mouth Neoplasms Neoplasm Invasiveness Adaptor Proteins Signal Transducing Cell Proliferation |
| Zdroj: | Shanghai kou qiang yi xue = Shanghai journal of stomatology. 28(1) |
| ISSN: | 1006-7248 |
| Popis: | This study was designed to investigate the effects of LASP1 on proliferation, metastasis, invasion, and cycle of oral squamous cell carcinoma cells and analyze the changes of IC50 in three antitumor drugs: cisplatin, apatinib and docetaxel.The correlation between LASP1 and survival rate and prognosis of patients with head and neck cancer were analyzed on the human protein atlas data. RT-PCR and Western blot were used to detect mRNA and protein expression of LASP1 in oral squamous cell carcinoma cell lines. LASP1 silenced HN30 stable transfectant cell line was constructed by lentivirus. CCK-8 assay was used to detect cell proliferation. Plate colony assay was used to detect cell clone formation ability. Transwell assay was used to detect cell migration and invasion ability. Flow cytometry was used to detect cell cycle changes. Oral squamous cell carcinoma metastases were established in nude mouse, the number of metastatic lung nodules was counted and stained with H-E. CCK-8 method was used to analyze the changes of IC50 in three antitumor drugs: cisplatin, apatinib and docetaxel. Statistical analysis was performed using SPSS 11.0 software package.LASP1 was closely related to the survival rate and prognosis of head and neck cancer. LASP1 promoted proliferation, colony formation, metastasis and invasion of oral squamous cell carcinoma cell line HN30, promoted G2/M phase transition of cell cycle, and significantly reduced the formation of lung metastasis in nude mice after silencing. There was significant correlation with docetaxel IC50 but no significant impact on cisplatin IC50 and aptatinib IC50.LASP1 enhances cell proliferation, plate cloning, metastasis and invasion, G2/M phase transition of cell cycle, promotes lung metastasis in nude mice and docetaxel resistance of oral squamous cell carcinoma cell line HN30. |
| Databáze: | OpenAIRE |
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