One-day pulsed-field gel electrophoresis protocol for rapid determination of emetic Bacillus cereus isolates

Autor:	Paulina S, Kaminska, Krzysztof, Fiedoruk, Dominika, Jankowska, Jacques, Mahillon, Karol, Nowosad, Ewa, Drewicka, Monika, Zambrzycka, Izabela, Swiecicka
Rok vydání:	2014
Předmět:	Foodborne Diseases Bacillus cereus Vomiting Depsipeptides Humans Food Contamination Electrophoresis Gel Pulsed-Field
Zdroj:	Electrophoresis. 36(7-8)
ISSN:	1522-2683
Popis:	Bacillus cereus, the Gram-positive and spore-forming ubiquitous bacterium, may cause emesis as the result of food intoxication with cereulide, a heat-stable emetic toxin. Rapid determination of cereulide-positive B. cereus isolates is of highest importance due to consequences of this intoxication for human health and life. Here we present a 1-day pulsed-field gel electrophoresis for emetic B. cereus isolates, which allows rapid and efficient determination of their genomic relatedness and helps determining the source of intoxication in case of outbreaks caused by these bacilli.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::52331978766b32f5862bc097fcefe7d5 https://pubmed.ncbi.nlm.nih.gov/25639850 Zobrazit plný text záznamu Plný text