Curcumin: a potential radio-enhancer in head and neck cancer
| Autor: | Avi, Khafif, Shahar, Lev-Ari, Akiva, Vexler, Itai, Barnea, Alex, Starr, Vicki, Karaush, Sasha, Haif, Rami, Ben-Yosef |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Mitogen-Activated Protein Kinase 1
Curcumin Mitogen-Activated Protein Kinase 3 Dose-Response Relationship Drug Cell Survival Anti-Inflammatory Agents Non-Steroidal Mice Nude Flow Cytometry ErbB Receptors Mice Cyclooxygenase 2 Head and Neck Neoplasms Cell Line Tumor Carcinoma Squamous Cell Animals Phosphorylation |
| Zdroj: | The Laryngoscope. 119(10) |
| ISSN: | 1531-4995 |
| Popis: | To investigate whether curcumin enhances the cytotoxic effect of radiotherapy in head and neck squamous cell carcinoma (HNSCC).HNSCC cell lines SCC-1, SCC-9, KB, as well as A431 cell line were treated with curcumin, irradiation, or their combination. Cell viability was evaluated by XTT assay. Cyclooxygenase-2 (COX-2), epithelial growth factor receptor (EGFR), and p-Erk1/2 were measured by Western blot analysis. CD-1 athymic nude mice with orthotopic implanted SCC-1 cells, were treated with control diet, curcumin containing diet, local single-dose radiation, or combination.Curcumin (IC50 range, 15-22 microM) and radiation inhibited cell viability in all cell lines were tested. The combination of curcumin and radiation resulted in additive effect. Curcumin decreased COX-2 expression and inhibited phosphorylation of EGFR in SCC-1 cells. In tumor-bearing mice the combination regimen showed a decrease in both tumor weight (25%, P = .09) and tumor size (15%, P = .23) compared to the nontreated mice.: Curcumin inhibited HNSCC cell growth and augmented the effect of radiation in vitro and in vivo. A possible mechanism is inhibition of COX-2 expression and EGFR phosphorylation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text