Protective role of Osthole on myocardial cell apoptosis induced by doxorubicin in rats
| Autor: | Hongdang, Xu, Yu, Han, Mengwei, Zhang, Min, Yan, Chuanyu, Gao |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Antibiotics
Antineoplastic Dose-Response Relationship Drug Caspase 3 Eukaryotic Initiation Factor-2 bcl-X Protein Cytochromes c NADPH Oxidases Apoptosis Antioxidants Cardiotoxicity Rats Sprague-Dawley Oxidative Stress Animals Newborn Coumarins Cytoprotection Doxorubicin Superoxides Animals Myocytes Cardiac Original Article Phosphorylation Cells Cultured NADP |
| Zdroj: | International journal of clinical and experimental pathology. 8(9) |
| ISSN: | 1936-2625 |
| Popis: | Objective: To explore the effect of Osthole on protecting myocardial cell apoptosis induced by doxorubicin during cardiac failure in rats. Methods: Myocardial cells isolated from the newborn SD rats were separated into three groups: cells treated with 1 μmol doxorubicin, cells treated with Osthole at three concentrations of 10, 20, and 40 μmol, cells treated neither with Osthole nor with doxorubicin were the control groups. Consequently, cell apoptosis of myocardial cells in each group was analyzed using TUNEL assay. Also, expressions of oxidase, NADPH, and ROS in myocardial cells were analyzed using different biological methods. Moreover, expressions of cell apoptosis associated proteins were analyzed using Western blotting. Results: Compared with the controls, the results showed that cells received Osthole and doxorubicin treatments performed high percentage of cell apoptosis, suggesting that Osthole could anesis myocardial cell apoptosis induced by doxorubicin (P |
| Databáze: | OpenAIRE |
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