| Autor: |
Maria, Dabrowska-Szponar, Janusz, Galiński |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Medycyna doswiadczalna i mikrobiologia. 55(2) |
| ISSN: |
0025-8601 |
| Popis: |
Usefulness of PCR--fingerprinting technic using ERIC 1 and ERIC 2 primers for epidemiologic analysis of Group B Streptococci was assessed. 120 strains isolated from various clinical samples were tested. Amplification reactions were carried out in automatic termocycler Poly Gen using two primers ERIC 1 and ERIC 2. Products of the amplification were subjected to horizontal electrophoresis in 1.5% agarose gel. Using primer ERIC 1 six DNA patterns were found. Patterns B and C were the most often encountered, containing 34.2% and 35.0% of strains. Less often pattern D occurred including 12.5% of strains. In case of primer ERIC 2 lower differentiation has been achieved, because only four DNA patterns has been found, three of them occurred with similar frequency 29.2% to 33.3%. Taking into consideration the patterns gained by means of two primers thirteen genotypes have been identified. Dominated two patterns CD and BC, comprising 30.0% and 24.1% Group B Streptococci. More rarely DA and BA occurred (10.8% and 8.3%). Remaining patterns make 0.8% to 6.7% strains. Among reference Group B Streptococci strains belonging to nine serologic types using two primers various genetic patterns were obtained. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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