Phytochemical Profiling, In Vitro and In Silico Anti-Microbial and Anti-Cancer Activity Evaluations and Staph GyraseB and
| Autor: | Hamdoon A, Mohammed, Riaz A, Khan, Atef A, Abdel-Hafez, Marwa, Abdel-Aziz, Eman, Ahmed, Shymaa, Enany, Sebaey, Mahgoub, Osamah, Al-Rugaie, Mansour, Alsharidah, Mohamed S A, Aly, Ahmed B M, Mehany, Mostafa M, Hegazy |
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| Rok vydání: | 2020 |
| Předmět: |
human topoisomerase-IIβ
HepG2 Zygophyllum chemical profile HCT-116 Phytochemicals Drug Evaluation Preclinical In Vitro Techniques Gas Chromatography-Mass Spectrometry Article Anti-Infective Agents Staph Gyrase B Candida albicans Staphylococcus pneumoniae Escherichia coli Humans Topoisomerase II Inhibitors Computer Simulation anti-biofilm Poly-ADP-Ribose Binding Proteins anti-cancer Plants Medicinal anti-microbial Mediterranean Region Plant Extracts Hep G2 Cells HCT116 Cells Antineoplastic Agents Phytogenic Molecular Docking Simulation Zygophyllum coccineum DNA Topoisomerases Type II DNA Gyrase Biofilms Pseudomonas aeruginosa MCF-7 Cells LC-ESI-TOF-MS Medicine Traditional Drug Screening Assays Antitumor MCF-7 |
| Zdroj: | Molecules |
| ISSN: | 1420-3049 |
| Popis: | Zygophyllum coccineum, an edible halophytic plant, is part of the traditional medicine chest in the Mediterranean region for symptomatic relief of diabetes, hypertension, wound healing, burns, infections, and rheumatoid arthritis pain. The current study aimed to characterize Z. coccineum phytoconstituents, and the evaluations of the anti-microbial-biofilm, and anti-cancers bioactivities of the plant’s mother liquor, i.e., aqueous-ethanolic extract, and its subsequent fractions. The in silico receptors interaction feasibility of Z. coccineum major constituents with Staph GyraseB, and human topoisomerase-IIβ (h-TOP-IIβ) were conducted to confirm the plant’s anti-microbial and anti-cancer biological activities. Thirty-eight secondary metabolites of flavonoids, stilbene, phenolic acids, alkaloids, and coumarin classes identified by LC-ESI-TOF-MS spectrometric analysis, and tiliroside (kaempferol-3-O-(6′′′′-p-coumaroyl)-glucoside, 19.8%), zygophyloside-F (12.78%), zygophyloside-G (9.67%), and isorhamnetin-3-O-glucoside (4.75%) were identified as the major constituents. A superior biofilm obliteration activity established the minimum biofilm eradication concentration (MBEC) for the chloroform fraction at 3.9–15.63 µg/mL, as compared to the positive controls (15.63–31.25 µg/mL) against all the microbial strains that produced the biofilm under study, except the Aspergillus fumigatus. The aqueous-ethanolic extract showed cytotoxic effects with IC50 values at 3.47, 3.19, and 2.27 µg/mL against MCF-7, HCT-116, and HepG2 cell-lines, respectively, together with the inhibition of h-TOP-IIβ with IC50 value at 45.05 ng/mL in comparison to its standard referral inhibitor (staurosporine, IC50, 135.33 ng/mL). This conclusively established the anti-cancer activity of the aqueous-ethanolic extract that also validated by in silico receptor-binding predicted energy levels and receptor-site docking feasibility of the major constituents of the plant’s extract. The study helped to authenticate some of the traditional phytomedicinal properties of the anti-infectious nature of the plant. |
| Databáze: | OpenAIRE |
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