| Autor: |
Ling-yun, Mu, Qin-mei, Wang, Yi-chang, Ni |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitologyparasitic diseases. 21(3) |
| ISSN: |
1000-7423 |
| Popis: |
To investigate the effect of daphnetin on superoxide dismutase (SOD) activity and DNA synthesis in P. falciparum in vitro.The effect of daphnetin, daphnetin-Fe complex and desferrioxamine B on SOD activity of P. falciparum (P.f) FCC1 in vitro was determined with a SOD test-kit. The level of DNA synthesis of P.f synchronized cultured in vitro at various developmental stages after treatment of daphnetin or desferrioxamine B was assayed by fluorescein Hoechest 33258.The total SOD activity decreased by 60% after daphnetin treatment while it only decreased by 22% if treated with desferrioxamine B. No effect on SOD activity of P.f treated with daphnetin-Fe complex was observed. The level of DNA synthesis of P.f trophozoites in synchronized in vitro culture was significantly lower than that of the control.Daphnetin lowered SOD activity and decreased DNA synthesis of P.f in vitro. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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