Physical markers for landmarking fluorescently stained gels that facilitate automated spot-picking

Autor:	V, Fehring, S, Wandschneider, M, Löhr
Rok vydání:	2001
Předmět:	Automation Pancreatic Juice Proteome Staining and Labeling Humans Proteins Spectrophotometry Ultraviolet Equipment Design Robotics Densitometry Specimen Handling
Zdroj:	Electrophoresis. 22(14)
ISSN:	0173-0835
Popis:	The quantitative comparison of spot patterns relies heavily on protein stains that do provide an appropriate dynamic range. Unfortunately most spot picking robot devices are still limited to nonfluorescent protein stains and the appropriate equipment is still quite expensive. These problems are solved by the application of a newly developed "GelMarker" that combines a spot picking robot device and a UV scanner. The "GelMarkers" are detectable in both the visible and UV range of light and permit the comparison of gel pictures taken by such different devices. The application of these "GelMarkers" together with the transformation of spot coordinates by using a "spot matching" procedure allows the automated excision of selected protein spots. The obtained picking accuracies are as good as those obtainable from visible stained gels due to the shape stability of the gels even over a longer time period.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::4ca2dbee6a2cd81d14ff28eddcfc2a7e https://pubmed.ncbi.nlm.nih.gov/11565786 Zobrazit plný text záznamu Plný text