Inhaled corticosteroids increase siglec-5/14 expression in sputum cells of COPD patients
| Autor: | P, Wielgat, R M, Mroz, A, Stasiak-Barmuta, P, Szepiel, E, Chyczewska, J J, Braszko, A, Holownia |
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| Rok vydání: | 2014 |
| Předmět: |
Neutrophils
Macrophages T-Lymphocytes Scopolamine Derivatives Sputum Antigens Differentiation Myelomonocytic Gene Expression Receptors Cell Surface Cell Separation Cholinergic Antagonists Bronchodilator Agents Immunophenotyping Androstadienes Pulmonary Disease Chronic Obstructive Adrenal Cortex Hormones Antigens CD Ethanolamines Formoterol Fumarate Lectins Fluticasone Humans Drug Therapy Combination Tiotropium Bromide Budesonide Adrenergic beta-2 Receptor Agonists |
| Zdroj: | Advances in experimental medicine and biology. 839 |
| ISSN: | 0065-2598 |
| Popis: | Recent studies show that several Siglec receptors, such as Siglec-8 and Siglec-14, may be important therapeutic targets in asthma and COPD. Siglecs are a family of lectins belonging to the immunoglobulin superfamily and recognize sialic acid residues of glycoproteins. Most of Siglecs have intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIM), implicating them in the suppression of immunoreceptor signaling. Siglec-5/14 may be involved in the negative regulation of innate immune responses. The aim of this study was to analyze Siglec-5/14 expression in induced sputum cells of COPD patients in the following treatment combinations: (1) a long-acting beta2-agonist, formoterol; (2) formoterol combined with a long-acting antimuscarinic agent, tiotropium; and (3) formoterol combined with an inhaled corticosteroid or formoterol combined with tiotropium and with an inhaled corticosteroid. Siglec expression was assessed in sputum cells by flow cytometry using a specific monoclonal antibody. Double staining of cells indicated that Siglec-5/14 is expressed in monocyte/macrophages and neutrophils, but not in lymphocytes. Siglec-5/14 expression was significantly higher in patients receiving combined therapy including inhaled corticosteroids compared with patients taking only formoterol or formoterol + tiotropium. Our results suggest that inhaled corticosteroids may exert beneficial or negative effects, depending on the patients' phenotype, through increased immunosuppressive Siglec-5 or immunoactivatory Siglec-14 receptors, respectively. |
| Databáze: | OpenAIRE |
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