[Biological safety investigation of the production of human insulin using genetically altered E. coli K-12 cells. 2. Plasmid transfer by mobilization and cointegrate formation under environmental conditions]

Autor:	M, Mieschendahl, K, Finkernagel, S, Schaffrath
Jazyk:	němčina
Rok vydání:	1993
Předmět:	Genes Bacterial Conjugation Genetic Pseudomonas Escherichia coli Temperature Insulin Fresh Water Gene Expression Regulation Bacterial Cloning Molecular Genetic Engineering Water Microbiology Culture Media Plasmids
Zdroj:	Zentralblatt fur Hygiene und Umweltmedizin = International journal of hygiene and environmental medicine. 193(5)
ISSN:	0934-8859
Popis:	Plasmid transfer by mobilization and cointegrate formation was tested with expression plasmids for human insulin in laboratory media and in river water samples. Plasmid pLIZP4 which carries a nic/bom sequence could only be mobilized after cloning of mobilization genes. Plasmid mobilization could be detected in laboratory media only, not in river water samples. The Nic/Bom plasmid pSW3 could be transferred by cointegrate formation in laboratory media at 30 degrees C and 37 degrees C, not at room temperature or in river water. The results allow the conclusion that no transfer of the plasmids pLIZP4 and pSW3 will take place by mobilization or cointegrate formation under environmental conditions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::47dabe6b1ef1fb7fb0abe9edb83fcc4a https://pubmed.ncbi.nlm.nih.gov/8476500 Zobrazit plný text záznamu