Live-Cell Assessment of Reactive Oxygen Species Levels Using Dihydroethidine

Autor: Sander, Grefte, Werner J H, Koopman
Rok vydání: 2021
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 2275
ISSN: 1940-6029
Popis: Reactive oxygen species (ROS) play an important role in cellular (patho)physiology. Empirical evidence suggests that mitochondria are an important source of ROS, especially under pathological conditions. Here, we describe a method for ROS measurement using dihydroethidium (HEt) and live-cell microscopy.
Databáze: OpenAIRE