| Autor: |
Lang, Yang, Yanfeng, Lin, Lanfen, Lu, Mei, Xue, Hui, Ma, Xuguang, Guo, Kaiying, Wang, Peihan, Li, Xinying, Du, Kezong, Qi, Peng, Li, Hongbin, Song |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Frontiers in Microbiology |
| ISSN: |
1664-302X |
| Popis: |
The emergence of carbapenem-resistant Enterobacteriaceae poses a significant threat to public health worldwide. Here, we reported a multidrug-resistant Escherichia coli strain with two different blaNDM–5-carrying plasmids from China. Illumina short-read and MinION long-read whole genome sequencing were performed. Genomic analysis found that one blaNDM–5 gene together with mphA was located on a 55-kb IncX3 plasmid, while the other blaNDM–5 gene was on a novel 68-kb IncFII plasmid. Susceptibility testing and quantitative reverse transcription PCR results further indicated that the transconjugants with the IncX3 plasmid exhibited higher-level carbapenem resistance and expression of blaNDM–5 than those with both plasmids or the IncFII plasmid. Two other β-lactamase genes (blaCTX–M–15 and blaOXA–1) were also detected on another 160-kb IncF plasmid. This is the first report of coexistence of two blaNDM–5-carrying plasmids in a single bacterial isolate, highlighting the genetic complexity of NDM-5 carbapenemase circulation, and the urgent need for continued active surveillance. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
