[Identification of genes induced to chondrocytes by nitric oxide]
| Autor: | A I, Nikolaev, V I, Dubovaia, D Iu, Litvinov, A B, Poltaraus, D S, Ivanov, A M, Amchenkova, A N, Narovlianskiĭ, A F, Panasiuk, V S, Prasolov, K T, Turpaev |
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| Rok vydání: | 2002 |
| Předmět: |
DNA
Complementary Dose-Response Relationship Drug Nitric Oxide Neoplasm Proteins Rats Isoenzymes Chondrocytes Gene Expression Regulation Proto-Oncogene Proteins c-bcl-2 Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases S-Nitrosoglutathione Animals Myeloid Cell Leukemia Sequence 1 Protein Matrix Metalloproteinase 3 Nitric Oxide Donors Rabbits Rats Wistar Cells Cultured In Situ Hybridization Gene Library |
| Zdroj: | Molekuliarnaia biologiia. 36(5) |
| ISSN: | 0026-8984 |
| Popis: | Nitric oxide (NO) acts as a short-lived paracrine factor and selectively activates transcription of certain genes. The spectrum of inducible genes was studied in primary chondrocytes. A cDNA library was obtained by subtraction hybridization with RNAs isolated from rabbit chondrocytes before and after treatment with nitrosoglutathione, an NO-generating agent. Some of the cloned cDNAs were homologous to known mammalian genes and human EST. NO-dependent transcriptional activation was demonstrated for the stromelysin 1 and cyclooxygenase 2 genes and, for the first time, for mcl1 coding for an apoptosis suppressor. |
| Databáze: | OpenAIRE |
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