| Autor: |
Kurt, Boonen, Wouter, De Haes, Joris, Van Houtven, Rik, Verdonck, Geert, Baggerman, Dirk, Valkenborg, Liliane, Schoofs |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1719 |
| ISSN: |
1940-6029 |
| Popis: |
In differential peptidomics, peptide profiles are compared between biological samples and the resulting expression levels are correlated to a phenotype of interest. This, in turn, allows us insight into how peptides may affect the phenotype of interest. In quantitative differential peptidomics, both label-based and label-free techniques are often employed. Label-based techniques have several advantages over label-free methods, primarily that labels allow for various samples to be pooled prior to liquid chromatography-mass spectrometry (LC-MS) analysis, reducing between-run variation. Here, we detail a method for performing quantitative peptidomics using stable amine-binding isotopic and isobaric tags. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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