| Autor: |
Nese Lortlar, Ünlü, Fulya Ekiz, Kanik, Elif, Seymour, John H, Connor, M Selim, Ünlü |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1571 |
| ISSN: |
1940-6029 |
| Popis: |
Protein microarrays are emerging tools which have become very powerful in multiplexed detection technologies. A variety of proteins can be immobilized on a sensor chip allowing for multiplexed diagnostics. Therefore, various types of analyte in a small volume of sample can be detected simultaneously. Protein immobilization is a crucial step for creating a robust and sensitive protein microarray-based detection system. In order to achieve a successful protein immobilization and preserve the activity of the proteins after immobilization, DNA-directed immobilization is a promising technique. Here, we present the design and the use of DNA-directed immobilized (DDI) antibodies in fabrication of robust protein microarrays. We focus on application of protein microarrays for capturing and detecting nanoparticles such as intact viruses. Experimental results on Single-particle interferometric reflectance imaging sensor (SP-IRIS) are used to validate the advantages of the DDI method. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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