Enzymatic activities in cultured human lymphocytes that dephosphorylate dolichyl pyrophosphate and dolichyl phosphate

Autor:	J F, Wedgwood, J L, Strominger
Rok vydání:	1980
Předmět:	Dolichol Phosphates Kinetics Bacitracin Polyisoprenyl Phosphates Humans Lymphocytes Phosphorus Radioisotopes Cells Cultured Phosphoric Monoester Hydrolases
Zdroj:	The Journal of biological chemistry. 255(3)
ISSN:	0021-9258
Popis:	Enzymatic activities which dephosphorylate dolichyl phosphate (Dol-P) and dolichyl pyrophosphate (Dol-P-P) have been observed in membranes from cultured human lymphocytes. Neither activity requires divalent metals. Dol-P phosphatase is inhibited by inorganic phosphate but not by other phosphate-containing compounds. Dol-P-P phosphatase is inhibited by bacitracin but not by phosphate-containing compounds including the methylene analogue of pyrophosphate. These reactions are similar to those previously found in the cycle of bacterial wall peptidoglycan biosynthesis. A chemical synthesis of [32P]Dol-P and [32P]Dol-P-P is reported.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::3485292ab7aee73a8badbb728b2620da https://pubmed.ncbi.nlm.nih.gov/6243292 Zobrazit plný text záznamu