Relationship of a mouse Sertoli cell line (MSC-1) to normal Sertoli cells
| Autor: | M P, McGuinness, C C, Linder, C R, Morales, L L, Heckert, J, Pikus, M D, Griswold |
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| Rok vydání: | 1994 |
| Předmět: |
Anti-Mullerian Hormone
Male Sertoli Cells Antigens Polyomavirus Transforming Recombinant Fusion Proteins Transferrin Mice Transgenic Regulatory Sequences Nucleic Acid Androgen-Binding Protein Growth Inhibitors Saposins Cell Line Rats Mice Testicular Hormones Clusterin Animals Receptors FSH RNA Messenger Cell Division Glycoproteins Molecular Chaperones |
| Zdroj: | Biology of reproduction. 51(1) |
| ISSN: | 0006-3363 |
| Popis: | Recently, a mouse Sertoli cell line (MSC-1) was established from transgenic mice carrying a fusion gene composed of human Müllerian inhibitory substance transcriptional regulatory sequences linked to the SV40 T-antigen gene. This cell line contained a morphologically heterogeneous population of cells that expressed characteristic Sertoli cell mRNAs such as transferrin, the beta-subunit for inhibin, and sulfated glycoprotein-2 (SGP-2). In this study, we compared various characteristics of MSC-1 cells to primary cultures of Sertoli cells from immature rats or adult mice. Our observations indicated that: 1) These cells were ultrastructurally similar to mouse Sertoli cells in culture; 2) MSC-1 cells expressed mRNA for androgen-binding protein (ABP) and SGP-1, but not the receptor for FSH; 3) The expression of SGP-2 mRNA and secretion of SGP-2 increased approximately 2-fold when cells were cultured at 41 degrees C, the nonpermissive temperature for the SV40 virus, while SGP-1 and transferrin mRNA levels and secretion were unaffected; 4) Proliferation of these cells was serum-dose dependent and temperature dependent and could be inhibited by incubating MSC-1 cells at 41 degrees C; 5) Proliferation was also significantly reduced after cells were incubated in the presence of dibutyryl cAMP (dbcAMP) for 6 days; 6) Fifteen subcultures produced from single MSC-1 cells displayed similar levels of mRNA expression for transferrin or SGP-1. Together these data indicate that the MSC-1 cell line is composed of a single cell type displaying numerous characteristics of Sertoli cells. This cell line may provide a unique source of tissue for studying various aspects of Sertoli cell behavior. |
| Databáze: | OpenAIRE |
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