| Autor: |
L W, Durani, F, Jaafar, J K, Tan, K, Tajul Arifin, Y A, Mohd Yusof, W Z, Wan Ngah, S, Makpol |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
La Clinica terapeutica. 166(6) |
| ISSN: |
1972-6007 |
| Popis: |
Tocotrienols have been known for their antioxidant properties besides their roles in cellular signalling, gene expression, immune response and apoptosis. This study aimed to determine the molecular mechanism of tocotrienol-rich fraction (TRF) in preventing cellular senescence of human diploid fibroblasts (HDFs) by targeting the genes in senescence-associated signalling pathways.Real time quantitative PCR (qRT-PCR) was utilized to evaluate the expression of genes involved in these pathways.Our findings showed that SOD1 and CCS-1 were significantly down-regulated in pre-senescent cells while CCS-1 and PRDX6 were up-regulated in senescent cells (p0.05). Treatment with TRF significantly down-regulated SOD1 in pre-senescent and senescent HDFs, up-regulated SOD2 in senescent cells, CAT in young HDFs, GPX1 in young and pre-senescent HDFs, and CCS-1 in young, pre-senescent and senescent HDFs (p0.05). TRF treatment also caused up-regulation of FOXO3A in all age groups of cells (p0.05). The expression of TP53, PAK2 and CDKN2A was significantly increased in senescent HDFs and treatment with TRF significantly down-regulated TP53 in senescent cells (p0.05). MAPK14 was significantly up-regulated (p0.05) in senescent HDFs while no changes was observed on the expression of JUN. TRF treatment, however, down-regulated MAPK14 in young and senescent cells and up-regulated JUN in young and pre-senescent HDFs (p0.05).TRF modulated the expression of genes involved in senescence-associated signalling pathways during replicative senescence of HDFs. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
