Stimulation-induced transient changes in neuronal activity, blood flow and N-acetylaspartate content in rat prefrontal cortex: a chemogenetic fMRS-BOLD study
| Autor: | Morris H, Baslow, Christopher K, Cain, Robert, Sears, Donald A, Wilson, Alvin, Bachman, Scott, Gerum, David N, Guilfoyle |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Neurons Aspartic Acid Brain Mapping Magnetic Resonance Spectroscopy Action Potentials Prefrontal Cortex Reproducibility of Results Magnetic Resonance Imaging Sensitivity and Specificity Article nervous system diseases Molecular Imaging Rats Rats Sprague-Dawley nervous system Cerebrovascular Circulation mental disorders Animals Blood Flow Velocity Magnetic Resonance Angiography |
| Zdroj: | NMR in biomedicine. 29(12) |
| ISSN: | 1099-1492 |
| Popis: | Brain activation studies in humans have shown the dynamic nature of neuronal N-acetylaspartate (NAA) and N-acetylaspartylglutamate (NAAG) based on changes in their magnetic resonance spectroscopy (MRS) signals in response to stimulation. These studies demonstrated that upon visual stimulation there was a focal increase in cerebral blood flow (CBF) and a decrease in NAA, or in the total of NAA and NAAG signals in the visual cortex, and that these changes were reversed upon cessation of stimulation. In the present study we have developed an animal model in order to explore the relationships between brain stimulation, neuronal activity, CBF and NAA. We use “Designer Receptors Exclusively Activated by Designer Drugs” (DREADDs) technology for site-specific neural activation, a local field potential electrophysiological method for measurement of changes in the rate of neuronal activity, functional MRS for measurement of changes in NAA, and a blood oxygenation level-dependent (BOLD) MR technique for evaluating changes in CBF. We show that stimulation of the rat prefrontal cortex using DREADDs results in (a) an increase in level of neuronal activity, (b) an increase in BOLD and (c) decrease in the NAA signal. These findings show for the first time the tightly coupled relationships between stimulation, neuron activity, CBF and NAA dynamics in brain, and also provide the first demonstration of the novel inverse stimulation-NAA phenomenon in an animal model. |
| Databáze: | OpenAIRE |
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