Cloning and characterization of a genomic probe for malignant catarrhal fever virus

Autor: L M, Shih, J M, Irving, Y C, Zee, R F, Pritchett
Rok vydání: 1988
Předmět:
Zdroj: American journal of veterinary research. 49(10)
ISSN: 0002-9645
Popis: A genomic probe specific for malignant catarrhal fever (MCF) virus was cloned by using purified viral DNA from MCF-virus strain WCll. Restriction endonuclease analysis of the purified viral DNA was used to identify the cloned viral genomic fragment. Dot blot hybridization by use of the genomic probe (pRP-5) indicated that the probe hybridized specifically with WCll-MCF virus, as well as with one other isolate of MCF-associated herpesvirus. Hybridization also was observed to a non-MCF virus strain of bovine herpesvirus.
Databáze: OpenAIRE