| Autor: |
A R, Ibragimov, S V, Shliapnikov, N A, Maĭsurian, E L, Arsen'eva, G T, Bogacheva |
| Rok vydání: |
1987 |
| Předmět: |
|
| Zdroj: |
Molekuliarnaia biologiia. 21(1) |
| ISSN: |
0026-8984 |
| Popis: |
We have analysed reassociated Ig molecules, containing heavy (H) or light (L) chains of Ig-B2 monoclonal antibody with human fibronectin binding activity and L of H chains of normal mouse serum immunoglobulin (Ig-NM). Examination of Ig-B2 idiotype expression in reassociated Ig indicated that 0.4% L-NM and 0.8% H-NM were able to restore Ig-B2 idiotype. The analysis of antigen binding capacity of reassociated Ig demonstrated, that only 4% H-NM created antigen binding site in complex with L-B2. We have determined the leading role of L-chain in creation of idiotype and binding site of Ig-B2. Selectivity of interaction between H and L chains is discussed. The results indicate, that not more than 4-6% of random H--L combinations produce functional Ig. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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