[Transposition of insertional sequence IS21 in E. coli cells activated by an exogenous promotor]

Autor: A N, Dubeĭkovskiĭ
Rok vydání: 1989
Předmět:
Zdroj: Molekuliarnaia genetika, mikrobiologiia i virusologiia. (1)
ISSN: 0208-0613
Popis: Transpositional activity of the IS21-element was found to be activated during its cloning in the pUC18 vector plasmid. The activated IS-element restores ability to transposition of the deficient IS21-element present in the same cell. Simultaneously with the activated transposition the increase in frequencies of precise exclusion of IS-element is registered. The activation of IS-element is concluded to be directed by exogenous promoter.
Databáze: OpenAIRE