Live Cell Imaging of Yeast Golgi Dynamics

Autor: Giancarlo, Costaguta, Gregory S, Payne, Lydia, Daboussi
Rok vydání: 2022
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 2557
ISSN: 1940-6029
Popis: Fluorescence imaging of live cells allows for the observation of dynamic processes inside cells in real time. Here we describe a strategy to image clathrin-coated vesicle dynamics in a single focal plane at the trans-Golgi network of the yeast Saccharomyces cerevisiae. This method can be readily adapted for live cell imaging of a diverse set of dynamic processes within cells.
Databáze: OpenAIRE