| Autor: |
Akira, Matsuda, Yuki, Shimizu, Teppei, Kanda, Akihiro, Ohnishi, Noritaka, Maeta, Masahiro, Miyabe, Kaori, Saeki, Yoshiki, Itoh |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Can J Vet Res |
| ISSN: |
1928-9022 |
| Popis: |
Some immortalized lens epithelial cell lines have been established and are useful for molecular analysis. The establishment of additional cell lines must, however, enable a variety of in-vitro examinations. The objective of this study was to establish a new canine lens epithelial cell line by isolating CLC-1 cells from the lens tissue of a dog with cataracts. In CLC-1 cells, transforming growth factor beta (TGF-β) treatment significantly decreased gene expression of an epithelial marker and elevated that of mesenchymal markers; these characteristics are similar to those of a human lens epithelial cell line. Interestingly, CLC-1 cells exhibited lower expression of an epithelial marker and higher expression of mesenchymal markers than an anterior lens capsule. These results suggest that CLC-1 cells were derived from a cell population that was committed to epithelial-mesenchymal transition in cataract lens tissue. In conclusion, CLC-1 cells could be useful for analyzing molecular pathogenesis in canine cataracts. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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