[Application of PCR in the diagnosis of B-type non-Hodgkin's lymphomas in cytological specimens from fine-needle aspiration]
| Autor: | A, Movilia, B, De Servi, A, Assi |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Hyperplasia
Lymphoma B-Cell Genes Immunoglobulin Biopsy Needle Gene Rearrangement B-Lymphocyte Heavy Chain Antibodies Monoclonal DNA Neoplasm Hodgkin Disease Neoplasm Proteins Diagnosis Differential DNA Nucleotidyltransferases Humans Lymph Nodes False Negative Reactions Lymphatic Diseases VDJ Recombinases |
| Zdroj: | Pathologica. 92(3) |
| ISSN: | 0031-2983 |
| Popis: | The application of PCR to detect monoclonality at the immunoglobulin heavy chain gene locus in FNA cytologic specimens was evaluated.Immunoglobulin heavy chain gene rearrangement analysis was performed by a nested PCR for the VDJ region on 27 lymph nodes FNA paraffin embedded by cell blocking technique specimens (1 suspicious of NOS lymphoma, 1 Hodgkin's disease, 6 B cell non Hodgkin's lymphomas, 10 reactive hyperplasias).A monoclonal band was seen in 13 of 17 suspicious or positive malignant cases. The other 4 specimens gave a policlonal pattern. Specimens from reactive lymph nodes produced policlonal bands in 8 cases, no product in 1 case and 1 specimen gave a monoclonal band. When needed, the results of PCR examinations were compared with the subsequent histologic evaluation of the same lymph-node.From our experience we can see that clonal analysis by PCR based IgH gene rearrangement analysis can be applied to FNA material and can be useful in diagnosis. |
| Databáze: | OpenAIRE |
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