| Autor: |
K A, Karlsson, B E, Samuelsson, G O, Steen |
| Rok vydání: |
1972 |
| Předmět: |
|
| Zdroj: |
Journal of lipid research. 13(2) |
| ISSN: |
0022-2275 |
| Popis: |
A pentose-containing cerebroside has been identified in the salt gland of the herring gull, using mass spectrometry of acetyl and trimethylsilyl derivatives. A detailed interpretation of the spectra allowed a conclusion concerning the major long-chain base (the C(20) homolog of sphingosine) and the major fatty acids (C(22)-C(25) 2-hydroxy fatty acids), using reference spectra of synthetic galactosylceramides. A six-membered glycose ring (aldopyranose) was demonstrated by mass spectrometry of the acetyl derivative of periodic acid-oxidized and sodium borodeuteride-reduced pentosylceramide. By gas-liquid chromatography and mass spectrometry of methanolysis products, the pentose was shown to be identical with xylose. The procedures were applied to 25-50 micro g of glycolipid. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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