| Autor: |
P K, Chakraborti, M J, Garabedian, K R, Yamamoto, S S, Simons |
| Rok vydání: |
1991 |
| Předmět: |
|
| Zdroj: |
The Journal of biological chemistry. 266(33) |
| ISSN: |
0021-9258 |
| Popis: |
Almost all modifications of the steroid binding domain of glucocorticoid receptors are known to cause a reduction or loss of steroid binding activity. Nonetheless, we now report that mutations of cysteine 656 of the rat receptor, which was previously suspected to be a crucial amino acid for the binding process, have produced "super" receptors. These receptors displayed an increased affinity for glucocorticoid steroids and a decreased relative affinity for cross-reacting steroids such as progesterone and aldosterone. The increased in vitro affinity of the super receptors was maintained in a whole cell bioassay. These results indicate that additional modifications of the glucocorticoid receptor, and probably the other steroid receptors, may further increase the binding affinity and/or specificity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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