[Analogs of nucleotides, modified by a sugar residue and pyrimidine base, in a DNA synthesis reaction, catalyzed by Thermus aquaticus DNA polymerase]

Autor:	L P, Savochkina, L B, Dĭachenko, M A, Lukin, L A, Aleksandrova
Rok vydání:	1992
Předmět:	Base Sequence Cell-Free System Nucleotides Molecular Sequence Data Carbohydrates DNA DNA-Directed DNA Polymerase Catalysis Substrate Specificity Pyrimidines Thymine Nucleotides Taq Polymerase Thermus Zidovudine Dideoxynucleotides
Zdroj:	Molekuliarnaia biologiia. 26(1)
ISSN:	0026-8984
Popis:	Substrate properties of dNTP analogues in the DNA synthesis reaction catalyzed by Thermus aquaticus DNA polymerase were studied. It was shown that most of dNTP analogues which were known as terminators of DNA synthesis of E. coli DNA polymerase I were able to terminate DNA synthesis catalyzed by Thermus aquaticus DNA polymerase. An interesting feature of Thermus aquaticus DNA polymerase was the ability to utilize 3'-azido-2',3'-dideoxythymidine triphosphate as terminating substrate. Relative efficiency of tested dNTP analogues incorporation into the DNA growing chain was estimated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::18f1e1ce0f5af9f62196e655b5f18fc8 https://pubmed.ncbi.nlm.nih.gov/1508169 Zobrazit plný text záznamu