Quantitative gene expression analysis in microdissected archival tissue by real-time RT-PCR

Autor:	K, Specht, T, Richter, U, Müller, A, Walch, M W, Höfler
Rok vydání:	2000
Předmět:	Receptor ErbB-2 Reverse Transcriptase Polymerase Chain Reaction Dissection Lasers Neoplasms Histological Techniques Gene Expression Humans RNA
Zdroj:	Journal of molecular medicine (Berlin, Germany). 78(7)
ISSN:	0946-2716
Popis:	Laser-assisted microdissection is a powerful tool for the analysis of morphologically defined cell populations. We report here that the combination of microdissection and real-time RT-PCR technologies together with an optimized RNA microscale extraction procedure allows the reproducible determination of gene expression levels in archival formalin-fixed, paraffin-embedded tissue samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::166df69165d98e94bc816c173bca410b https://pubmed.ncbi.nlm.nih.gov/11043388 Zobrazit plný text záznamu Full text from SpringerLink