Increased alpha3-fucosylation of alpha1-acid glycoprotein in Type I diabetic patients is related to vascular function
| Autor: | D C, Poland, C G, Schalkwijk, C D, Stehouwer, C A, Koeleman, B, van het Hof, W, van Dijk |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Inflammation Male Glycosylation Adolescent Statistics as Topic Oligosaccharides Orosomucoid Middle Aged Chromatography Ion Exchange Fucosyltransferases Diabetes Mellitus Type 1 Lectins Carbohydrate Conformation Concanavalin A Albuminuria Humans Female Sialyl Lewis X Antigen Diabetic Angiopathies Aged Fucose |
| Zdroj: | Glycoconjugate journal. 18(3) |
| ISSN: | 0282-0080 |
| Popis: | Diabetic mellitus is attended by the development of endothelial dysfunction which is suggested to be accompanied with a chronic low-degree of inflammation. During a chronic hepatic inflammatory response, specific changes in glycosylation of the acute phase protein alpha1-acid glycoprotein (AGP) can be detected. In this report we studied the changes in glycosylation of AGP in more detail and evaluated the relation between a change in glycosylation of AGP and urinary albumin secretion in Type I diabetic patients. The glycosylation of AGP, studied by crossed affinity immunoelectrophoresis (CAIE) and high pH anion exchange chromatography with pulse amperometric detection (HPAEC-PAD), showed an increase in alpha3-fucosylation. Staining with an antibody against sialyl Lewis(x) (sLe(x)) implied that part of the alpha3-fucosylation was present in a sLe(x)-conformation. In the group of Type I diabetic patients with increased urinary albumin excretion, a significant increase in alpha3-fucosylation of AGP (p0.0005) could be detected. Therefore, the increased alpha3-fucosylation of AGP can be used as an additional marker for the development of vascular complications in Type I diabetic patients. |
| Databáze: | OpenAIRE |
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