Transport of GM1 and GM1 inner ester across an in vitro model of the blood-brain barrier

Autor:	J M, Wells, R F, Ventura, P B, Eisenhauer, D C, McKenna, R E, Fine, M D, Ullman
Rok vydání:	1996
Předmět:	Kinetics Blood-Brain Barrier Astrocytes Models Neurological Animals Cattle Esters Endothelium G(M1) Ganglioside Hydrogen-Ion Concentration In Vitro Techniques Rats Tight Junctions
Zdroj:	Neuroscience letters. 217(2-3)
ISSN:	0304-3940
Popis:	Gangliosides, especially GM1, attenuate the in vivo damage caused by various neurotoxins. The chemically neutral inner ester of GM1 may be a better cytoprotective agent against some neurotoxins than the parent GM1 compound, because it may cross the blood-brain barrier (BBB) more easily than the anionic GM1. Using an in vitro bovine brain endothelial cell model of the BBB, we show the inner ester more readily transverse the tight junction barrier of this model than does GM1. Further, it is demonstrated that the GM1 inner ester is stable for several hours at pH values between 7.0 and 8.2 at 37 degrees C. Finally, the results illustrate that the BBB model may be useful for testing other gangliosides and their various derivatives for increased ability to cross the BBB.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::0cc82af12b37f930e328d9cf0ba68c76 https://pubmed.ncbi.nlm.nih.gov/8916087 Zobrazit plný text záznamu Full Text from ScienceDirect