| Autor: |
Maria M, González, Luis, Sarmiento, Jhon Carlos, Castaño, Alejandra M, Giraldo, Alexander, Salazar, Nini J, Muñoz |
| Rok vydání: |
2006 |
| Předmět: |
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| Zdroj: |
Revista de salud publica (Bogota, Colombia). 8 |
| ISSN: |
0124-0064 |
| Popis: |
Implementing environmental surveillance for poliovirus circulating from wastewater.Six wastewater samples were collected from each final site within a wastewater collection system in Armenia, Quindío. Virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent. Polioviruses were identified by polymerase chain reaction assay with group-specific, serotype-specific and Sabin vaccine-specific primers.It was confirmed that the viral detection method is highly efficient. Poliovirus was detected in 5 of 6 (83%) samples collected. Serotype-specific identification revealed the presence of type 1 and type 3 polioviruses in the samples. All of them were of the vaccine type, which provided evidence regarding the non-circulation of wild poliovirus in the country.Wastewater surveillance can be a sensitive tool for detecting poliovirus circulation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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