Estrogen receptors are identified in the glioblastoma cell line U138MG

Autor:	K K, Leslie, D, Keefe, S, Powell, F, Naftolin
Rok vydání:	1994
Předmět:	Receptors Estrogen Transcription Genetic Karyotyping Tumor Cells Cultured Humans Flow Cytometry Glioblastoma Ligands Receptors Progesterone Transfection Polymerase Chain Reaction Sensitivity and Specificity
Zdroj:	Journal of the Society for Gynecologic Investigation. 1(3)
ISSN:	1071-5576
Popis:	The antiestrogen tamoxifen has been found to be effective in decreasing glioblastoma cell proliferation, but the mechanism underlying this effect and whether it is through the estrogen receptor (ER) is controversial. The objective of this study was to determine whether ERs are present in three human glioblastoma cell lines--HS683, U138MG, and JHN J889H--using the most sensitive techniques available.Ligand binding and flow cytometry were employed to identify estrogen and progesterone receptors. The reverse transcriptase-polymerase chain reaction was used to identify ER mRNA, and a novel reporter gene transfection assay demonstrated that the ER was capable of activating gene transcription.U138MG glioblastoma cells contain ERs that are capable of increasing gene transcription in response to estradiol. No ERs were found in HS683 or JHN J889H cells.Tamoxifen may be acting through the ER in some glioblastoma cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::04625764d7fb83b858571f31f3268ae6 https://pubmed.ncbi.nlm.nih.gov/9419778 Zobrazit plný text záznamu Full text from SpringerLink