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Objective To observe the effects of force signals and inflammatory cytokines on the expressions of functional proteins during the differentiation of periodontal ligament cells(PDLCs) into osteoclasts. Methods The caries-free premolars that needed to be removed for orthodontic treatment were collected,human periodontal ligament cells were cultured in vitro.Human PDLCs were exposed to inflammatory cytokines including interleukin(IL)-1β,-6,-23,and tumor necrosis factor alpha(TNF-α). Cyclicmechanical tension with a maximum 5% elongation for different durations(0,2,4,8,12,and 24 hours) were applied. Then the expressions of signaling molecules related to osteoclastogenesis(OPG) and receptor activated nuclear factor κB ligand(RANKL) were determined at protein levels by Western blotting. Results Inflammatory cytokines improved the expressions of osteoclastgenesis regulators in hPDLCs,while cyclic-tension force reduced their expressions. However,the combined effect of inflammatory cytokines and cyclic-tension force resulted in high expressions of osteoclastgenesis regulators. Conclusion Inflammatory cytokines can promote the expressions of the osteoclastgenic factors,which can not be offset by cyclic-tension force.目的 观察力信号和炎症因子在牙周膜细胞向破骨细胞分化过程中对功能蛋白表达的影响。方法 收集因正畸治疗需要拔除的无龋前磨牙,体外培养人牙周膜细胞,分别对其施加5%基底型变量的周期性牵张力和白细胞介素(IL)-1β、-6、-23和肿瘤坏死因子(TNF)α等炎症因子刺激,Western blot检测施加刺激0、2、4、8、12、24 h后骨代谢相关调控因子骨保护素(OPG)和细胞因子κB受体激动剂配体(RANKL)的蛋白表达情况。结果 炎症因子对牙周膜细胞破骨向因子表达有促进作用,而周期性牵张力能够抑制破骨调控因子的表达,两者共同作用时破骨向因子呈现高表达状态。结论 炎症因子对破骨向调控因子有促进作用,而周期性牵张力抑制破骨的作用无法抵消这一过程。. |
